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The fourth tab
is used to overlay iso-contour lines onto the images. A histogram of the pixel values is shown, and the value at which the iso-contour lines are drawn can be adjusted by entering a numerical value, or by dragging the marker highlighted in the graphics above. The selection on the right allows to choose among several colors for the contour lines.
Per default, the iso-contour lines are shown as long as the iso-contour tab is selected. If another tab is selected, they disappear. To make them appear permanently in the images the pin button 
can be fixed to 
.